Datos fisiológicos basales y valorización enzimática de residuos pesqueros para la nutrición de hembras de Odontesthes argentinensis
DOI:
https://doi.org/10.47193/mafis.4012027010101Palabras clave:
Peptidasas, lipasas, factores antinutricionales, peces, harina de soja, residuos pesquerosResumen
Este estudio investigó los patrones de almacenamiento de energía en hembras silvestres de Odontesthes argentinensis capturadas durante el otoño en aguas costeras de Mar del Plata (Argentina) para evaluar su idoneidad como reproductoras, y exploró estrategias de alimentación innovadoras basadas en enzimas para mejorar la calidad del alimento, la utilización de nutrientes y el manejo nutricional de los reproductores de esta especie. Los análisis bioquímicos mostraron que el hígado fue el principal órgano de almacenamiento de lípidos y proteínas, mientras que los perfiles de ácidos grasos fueron coherentes con una estrategia reproductiva basada en reservas corporales (capital breeding) asociada a la reproducción estacional. Sin embargo, dado que este estudio se basó en un único muestreo estacional y careció de clasificación de estadios ováricos, índices gonadosomáticos o hepatosomáticos y series temporales, estos hallazgos deben interpretarse como evidencia preliminar y no considerarse suficientes para clasificar definitivamente la estrategia reproductiva. Para mejorar el valor nutricional de la harina de soja, el tratamiento enzimático con peptidasas de Urophycis brasiliensis en condiciones ácidas (pH 2) redujo los compuestos antinutricionales y preservó las moléculas bioactivas, mejorando así la calidad proteica. Además, la combinación in vitro de extractos de peptidasas exógenas provenientes de residuos pesqueros con enzimas digestivas de O. argentinensis aumentó la actividad proteolítica, lo que sugiere interacciones sinérgicas que justifican su evaluación en futuros ensayos de alimentación. En conjunto, estos hallazgos aportan información fisiológica y nutricional que puede contribuir al desarrollo de estrategias de alimentación para hembras de O. argentinensis. Asimismo, el uso de residuos pesqueros como fuente de enzimas digestivas representa un enfoque biotecnológico prometedor para mejorar la digestibilidad del alimento y promover prácticas acuícolas más sostenibles. No obstante, se requieren estudios adicionales que evalúen la digestibilidad, el desempeño de los peces, los rendimientos de extracción, la seguridad, la viabilidad económica y los impactos ambientales para corroborar estos beneficios potenciales.
Descargas
Referencias
Alarcón F, García-Carreño F, Navarrete del Toro MA. 2001. Effect of plant protease inhibitors on digestive proteases in two fish species, Lutjanus argentiventris and L. novemfasciatus. Fish Physiol Biochem. 24: 179-189. DOI: https://doi.org/10.1023/A:1014079919461
Alarcón FJ, Moyano FJ, Díaz M. 1999. Effect of inhibitors present in protein sources on digestive proteases of juvenile sea bream (Sparus aurata). Aquat Living Resour. 12 (4): 233-238. DOI: https://doi.org/10.1016/S0990-7440(00)86633-4
Albanesi C, Méndez E, González-Castro M, Michiels MS. 2025. Digestive, metabolic, and morphological adaptations of Odontesthes argentinensis to estuarine and marine environments: insights into phenotypic plasticity and ecological differentiation. Estuar Coast Shelf Sci. 324: 109446. DOI: https://doi.org/10.1016/j.ecss.2025.109446
Albanesi C, Méndez E, Toraño JC, Michiels MS. 2026. Integrated digestive and metabolic traits underlie trophic differentiation in juvenile fish assemblages. J Fish Biol. DOI: https://doi.org/10.1111/jfb.70594
Anson ML. 1938. The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin. J Gen Physiol. 22 (1): 79. DOI: https://doi.org/10.1085/jgp.22.1.79
[AOAC] Association of Official Agricultural Chemists. 1995. Official methods of analysis. 16th ed. Washington: Association of official analytical chemists.
Bou M, Berge GM, Baeverfjord G, Sigholt T, Østbye TK, Romarheim OH, Ruyter B. 2017. Requirements of n-3 very long-chain PUFA in Atlantic salmon (Salmo salar L): effects of different dietary levels of EPA and DHA on fish performance and tissue composition and integrity. Br J Nutr. 117 (1): 30-47. DOI: https://doi.org/10.1017/S0007114516004396
Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem. 72 (1-2): 248-254. DOI: https://doi.org/10.1016/0003-2697(76)90527-3
Bonadero MC, Laitano MV, del Valle JC, Fernández-Gimenez AV. 2022. Marine sources for biotechnology: preliminary digestive enzymes assessment of seven decapod species from the southwest Atlantic. Reg Stud Mar Sci. 55: 102515. DOI: https://doi.org/10.1016/j.rsma.2022.102515
Chen H, Muramoto K, Yamauchi F. 1995. Structural analysis of antioxidative peptides from soybean β-conglycinin. J Agric Food Chem. 43: 574-578. DOI: https://doi.org/10.1021/jf00051a004
Chen Y, Xu Z, Zhang C, Kong X, Hua Y. 2014. Heat-induced inactivation mechanisms of Kunitz trypsin inhibitor and Bowman-Birk inhibitor in soymilk processing. Food Chemistry. 154: 108-116. DOI: https://doi.org/10.1016/j.foodchem.2013.12.092
Chen Z, Fei S, Liu C, Duan Y, Liu H, Han D, Jin J, Yang Y, Zhu X, Xie S. 2023. Compared to fishmeal, dietary soybean meal improves the reproductive performance of female yellow catfish (Pelteobagrus fulvidraco) broodstock. Aquac Nutr. 2023: 6240803. DOI: https://doi.org/10.1155/2023/6240803
Coscueta ER, Amorim MM, Voss GB, Nerli BB, Picó GA, Pintado ME. 2016. Bioactive properties of peptides obtained from Argentinian defatted soy flour protein by Corolase PP hydrolysis. Food Chem. 198: 36-44. DOI: https://doi.org/10.1016/j.foodchem.2015.11.068
del Valle JC, Zanazzi AN, Rodriguez YE, Haran NS, Laitano MV, Mallo JC, Fernández-Gimenez AV. 2022. Morphological changes, peptidase activity, and effects of exogenous enzymes in the early ontogeny of Nile tilapia, Oreochromis niloticus. Aquac Int. 30 (4): 1645-1658. DOI: https://doi.org/10.1007/s10499-022-00932-5
Deng Z, Duarte ME, Kim SY, Hwang Y, Kim SW. 2023. Comparative effects of soy protein concentrate, enzyme-treated soybean meal, and fermented soybean meal replacing animal protein supplements in feeds on growth performance and intestinal health of nursery pigs. J Anim Sci Biotechnol. 14 (1): 89. DOI: https://doi.org/10.1186/s40104-023-00888-3
Díaz AC, Velurtas SM, Espino ML, Fenucci JL. 2014. Effect of dietary astaxanthin on free radical scavenging capacity and nitrite stress tolerance of postlarvae shrimp, Pleoticus muelleri. J Agric Food Chem. 62 (51): 12326-12331. DOI: https://doi.org/10.1021/jf503754q
Dubois M, Gilles KA, Hamilton JK, Rebers PT, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Anal Chem. 28 (3): 350-356. DOI: https://doi.org/10.1021/ac60111a017
Erdaw MM, Beyene WT. 2018. Anti-nutrients reduce poultry productivity: influence of trypsin inhibitors on pancreas. Asian J Poult Sci. 12 (1): 14-24. DOI: https://doi.org/10.3923/ajpsaj.2018.14.24
Friedman IS, Behrens LA, Pereira NA, Contreras E, Fernández-Gimenez AV. 2022. Digestive proteases from fish processing wastes: their partial characterization and comparison. J Fish Biol. 100: 150-160. DOI: https://doi.org/10.1111/jfb.14929
Führ F, Tesser MB, Rodrigues RV, Pedron J, Romano LA. 2016. Artemia enriched with hydrolyzed yeast improves growth and stress resistance of marine pejerrey Odontesthes argentinensis larvae. Aquaculture. 450: 173-181. DOI: https://doi.org/10.1016/j.aquaculture.2015.07.018
Fuiman LA, Perez KO. 2015. Metabolic programming mediated by an essential fatty acid alters body composition and survival skills of a marine fish. Proceedings of the Royal Society B: Biological Sciences. 282 (1819): 20151414. DOI: https://doi.org/10.1098/rspb.2015.1414
García-Carreño FL. 1992. The digestive proteases of langostilla (Pleuroncodes planipes, Decapoda): their partial characterization, and the effect of feed on their composition. Comp Biochem Physiol B: Biochem Mol Biol. 103: 575-578. DOI: https://doi.org/10.1016/0305-0491(92)90373-Y
González-Castro M, Rosso JJ, Delpiani SM. 2019. Inferring boundaries among fish species of the new world silversides (Atherinopsidae; genus Odontesthes): new evidences of incipient speciation between marine and brackish populations of Odontesthes argentinensis. Genetica. 147: 217-229. DOI: https://doi.org/10.1007/s10709-019-00066-2
González-Zamorano M, Navarrete del Toro MA, García-Carreño FL. 2013. Exogenous proteinases as feed supplement for shrimp: in vitro evaluation. Aquac Nutr. 19 (5): 731-740. DOI: https://doi.org/10.1111/anu.12020
Guidi C, Baigún CRM, Ginter LG, Soricetti M, Rivas FG, Morawicki S, Quezada F, Bazzani, JL, Solimano PJ. 2021. Characteristics, preferences and perceptions of recreational fishers in northern Patagonia, Argentina. Reg Stud Mar Sci. 45: 101828. DOI: https://doi.org/10.1016/j.rsma.2021.101828
Hara A, Hiramatsu N, Fujita T. 2016. Vitellogenesis and choriogenesis in fishes. Fish Sci. 82: 187-202. DOI: https://doi.org/10.1007/s12562-015-0957-5
Hernandez de Dios MA, Tovar-Ramírez D, Maldonado-García D, Galaviz-Espinoza MA, Spanopoulos Zarco M, Maldonado-García MC. 2022. Functional additives as a boost to reproductive performance in marine fish: A review. Fishes. 7 (5): 262. DOI: https://doi.org/10.3390/fishes7050262
Horn MH, Gawlicka A, German DP, Logothetis E, Cavanagh J, Boyle K. 2006. Structure and function of the stomachless digestive system in three related species of New World silverside fishes (Atherinopsidae) representing herbivory, omnivory, and carnivory. Mar Biol. 149: 1237-1245. DOI: https://doi.org/10.1007/S00227-006-0281-9
Izquierdo MS, Fernandez-Palacios H, Tacon AGJ. 2001. Effect of broodstock nutrition on reproductive performance of fish. Aquaculture. 197 (1-4): 25-42. DOI: https://doi.org/10.1016/S0044-8486(01)00581-6
Katan T, Xue X, Caballero-Solares A, Taylor RG, Rise ML, Parrish CC. 2020. Influence of dietary long-chain polyunsaturated fatty acids and ω6 to ω3 ratios on head kidney lipid composition and expression of fatty acid and eicosanoid metabolism genes in Atlantic salmon (Salmo salar). Front Mol Biosci. 7: 602587. DOI: https://doi.org/10.3389/fmolb.2020.602587
Le HT, Shao X, Krogdahl Å, Kortner TM, Lein I, Kousoulaki K, Sæle Ø. 2019. Intestinal function of the stomachless fish, ballan wrasse (Labrus bergylta). Front Mar Sci. 6: 140. DOI: https://doi.org/10.3389/fmars.2019.00140
Liang Q, Yuan M, Xu L, Lio E, Zhang F, Mou H, Secundo. 2022. Application of enzymes as a feed additive in aquaculture. Mar Life Sci Technol. 4 (2): 208-221. DOI: https://doi.org/10.1007/s42995-022-00128-z
Llompart FM, Colautti DC, Maiztegui T, Cruz-Jiménez AM, Baigún CRM. 2013. Biological traits and growth patterns of pejerrey Odontesthes argentinensis. J Fish Biol. 82 (2): 458-474. DOI: https://doi.org/10.1111/j.1095-8649.2012.03494.x
Logothetis E, Horn M, Dickson K. 2001. Gut morphology and function in Atherinops affinis (Teleostei: Atherinopsidae), a stomachless omnivore feeding on macroalgae. J Fish Biol. 59: 1298-1312. DOI: https://doi.org/10.1111/J.1095-8649.2001.TB00193.X
López S, Militelli MI, Riestra CM, Macchi GJ. 2025. Energy allocation strategy during the reproductive cycle of Patagonian grouper (Acanthistius patachonicus) from the southwestern Atlantic Ocean. Mar Fish Sci. 38 (3): 375-388. DOI: https://doi.org/10.47193/mafis.3832025010704
Ma J, Su K, Chen M, Wang S. 2023. Study on the antioxidant activity of peptides from soybean meal by fermentation based on the chemical method and AAPH-induced oxidative stress. Food Sci Nutr. 11: 6634-6647. DOI: https://doi.org/10.1002/fsn3.3612
Méndez E, Albanesi C, Michiels MS, López-Mañanes A, González-Castro M. 2024. Analyses of body condition and digestive/metabolic parameters of Odontesthes argentinensis (Atherinopsidae) from Mar Chiquita Coastal Lagoon (Argentina) during different phases of ovarian development. Neotrop Ichthyol. 22 (3): e230139. DOI: https://doi.org/10.1590/1982-0224-2023-0139
Méndez E, Albanesi C, Michiels MS, López Mañanes A, González-Castro M. 2026. Metabolic flexibility and seasonal physiological responses of the resident silverside Odontesthes argentinensis in a southwestern Atlantic coastal lagoon. J Fish Biol. 1-10. DOI: https://doi.org/10.1111/jfb.70482
Nolasco-Soria H, Moyano-López F, Vega-Villasante F, Del Monte A, Espinoza-Chaurand D, Gisbert E. 2018. Lipases and phospholipases: methods and protocols. In: Sandoval G, editor. Methods in molecular biology. Totowa: Springer, Humana Press. p. 139-167.
Rahman MS, Riad HM, Mahmud MS, Fatema MK, Shahjahan M. 2020. Farmers’ perception on quality of fish feed, brood stock and fingerling produced in commercial fish farms of Bangladesh. Bangladesh J Fish. 32 (1): 141-147. DOI: https://doi.org/10.52168/bjf.2020.32.16
R Core Team. 2023. R: A language and environment for statistical computing. R Foundation for Statistical Computing. https://www.r-project.org/.
Rodrigues KA, Leonarduzzi E, Militelli M., Macchi GJ. 2025. Condition and energy allocation during the reproductive cycle of the Patagonian redfish Sebastes oculatus from the Argentine continental shelf. Mar Fish Sci. 38 (4): 575-589. DOI: https://doi.org/10.47193/mafis.3842025011002
Rodriguez YE, Pereira NA, Haran NS, Mallo JC, Fernández-Gimenez AV. 2017. A new approach to fishery waste revalorization to enhance Nile tilapia (Oreochromis niloticus) digestion process. Aquac Nutr. 23: 1351-1361. DOI: https://doi.org/10.1111/anu.12510
Rodriguez YE, Pereira NA, Laitano MV, Moreno P, Fernández-Gimenez AV. 2021. Exogenous proteases from seafood processing waste as functional additives in rainbow trout aquaculture. Aquac Res. 52 (9): 4350-4361. DOI: https://doi.org/10.1111/are.15272
Rodríguez-Barreto D, Jerez S, Cejas JR, Martín MV, Acosta NG, Bolaños A, Lorenzo A. 2012. Comparative study of lipid and fatty acid composition in different tissues of wild and cultured female broodstock of greater amberjack (Seriola dumerili). Aquaculture. 360: 1-9. DOI: https://doi.org/10.1016/j.aquaculture.2012.07.013
Sabetian A, Hoang LH, Zhang J, White WL, Chen T, Wang H, Lilkendey J. 2025. Fatty acid biomarkers reveal maternal energy allocation strategies in spawning Snapper (Chrysophrys auratus). New Zealand J Mar Freshw Res. 59 (5): 1117-1130. DOI: https://doi.org/10.1080/00288330.2025.2464048
Schismenou E, Chatzifotis S, Tsiaras K, Somarakis S. 2024. Anchovy and sardine condition and energy content in the North Aegean Sea (eastern Mediterranean) in relation to their contrasting reproductive strategies. J Fish Biol. 105 (4): 1178-1188. DOI: https://doi.org/10.1111/jfb.15872
Sila A, Bougatef A. 2016. Antioxidant peptides from marine by-products: Isolation, identification and application in food systems. A review. J Funct Foods. 21: 10-26. DOI: https://doi.org/10.1016/j.jff.2015.11.007
Sissener NH, Araujo P, Sæle Ø, Rosenlund G, Stubhaug I, Sanden M. 2020. Dietary 18: 2n-6 affects EPA (20: 5n-3) and ARA (20: 4n-6) content in cell membranes and eicosanoid production in Atlantic salmon (Salmo salar L.). Aquaculture 522: 735098. DOI: https://doi.org/10.1016/j.aquaculture.2020.735098
Soengas JL, Barciela P, Aldegunde M. 1995. Variations in carbohydrate metabolism during gonad maturation in female turbot (Scophthalmus maximus). Mar Biol. 123: 11-18. DOI: https://doi.org/10.1007/BF00350318
Soltan NM, Soaudy MR, Abdella MM, Hassaan MS. 2023. Partial dietary fishmeal replacement with mixture of plant protein sources supplemented with exogenous enzymes modify growth performance, digestibility, intestinal morphology, haemato-biochemical and immune responses for Nile tilapia, Oreochromis niloticus. Anim Feed Sci Technol. 299: 115642. DOI: https://doi.org/10.1016/j.anifeedsci.2023.115642
Sun K, Gao M, Wang Y, Li, XR, Wang P, Wang B. 2022. Antioxidant peptides from protein hydrolysate of marine red algae Eucheuma cottonii: Preparation, identification and cytoprotective mechanisms on H2O2 oxidative damaged HUVECs. Front Microbiol. 13: 791248. DOI: https://doi.org/10.3389/fmicb.2022.791248
Thiex NJ, Manson H, Anderson S, Persson JÅ. 2002. Determination of crude protein in animal feed, forage, grain, and oilseeds by using block digestion with a copper catalyst and steam distillation into boric acid: collaborative study. J AOAC Int. 85 (2): 309-317. DOI: https://doi.org/10.1093/jaoac/85.2.309
Thompson GA, Volpedo AV. 2018. Diet composition and feeding strategy of the New World silverside Odontesthes argentinensis in a temperate coastal area (South America). Mar Coast Fish. 10 (1): 80-88. DOI: https://doi.org/10.1002/mcf2.10005
Toledo-Cuevas E, Chávez-Sánchez MC, Herrera-Vargas MA, Abad Rosales SM, Cortés Ortiz E, Strüssmann CA, Raggi L. 2024. Early anatomical development of the digestive system and high intestinal digestive enzyme activity in two silverside species, Odontesthes bonariensis and O. hatcheri (Atherinomorpha: Atherinopsidae). Lat Am J Aquat Res. 52 (3): 368-382. DOI: https://doi.org/10.3856/vol52-issue3-fulltext-3155
Vagadia BH, Vanga SK, Raghavan V. 2017. Inactivation methods of soybean trypsin inhibitor - a review. Trends Food Sci Technol. 64: 115-125. DOI: https://doi.org/10.1016/j.tifs.2017.02.003
Volkoff H, London S. 2018. Nutrition and reproduction in fish. In: Skinner MA, editor. Encyclopedia of reproduction. New York: Academic Press. p. 743-748.
Weng L, Wang Z, Zhuang W, Yang T, Xu X, Liu J, Liu J, Xu Z, Chen R, Wang Q, et al. 2023. Effect of replacing fish meal using fermented soybean meal on growth performance, intestine bacterial diversity, and key gene expression of largemouth bass (Micropterus salmoides). Fermentation. 9 (6): 520. DOI: https://doi.org/10.3390/fermentation9060520
Wu J, Muir AD. 2010. Isoflavone during protease hydrolysis of defatted soybean meal. Food Chem. 118 (2): 328-332. DOI: https://doi.org/10.1016/j.foodchem.2009.04.129
Xu H, Meng X, Wei Y, Ma Q, Liang M, Turchini GM. 2022. Arachidonic acid matters. Reviews in Aquaculture. 14 (4): 1912-1944. DOI: https://doi.org/10.1111/raq.12679
Yilmaz O, Sullivan CV, Bobe J, Norberg B. 2024. The role of multiple vitellogenins in early development of fishes. Gen Comp Endocrinol. 351: 114479. DOI: https://doi.org/1016/j.ygcen.2024.114479
Zhang Q, Li F, Guo M, Qin M, Wang J, Yu H, Xu J, Liu Y, Tong T. 2023. Growth performance, antioxidant and immunity capacity were significantly affected by feeding fermented soybean meal in juvenile coho salmon (Oncorhynchus kisutch). Animals. 13 (5): 945. DOI: https://doi.org/10.3390/ani13050945
Zhao Z, Zhao Q, Wan H, Wei L, Wang S, Li S, Wang Z. 2023. Integrated transcriptomic and metabolomic analyses identify key factors in the vitellogenesis of juvenile Sichuan bream (Sinibrama taeniatus). Front Mar Sci. 10: 1243767. DOI: https://doi.org/10.3389/fmars.2023.1243767
Zheng CC, Wu JW, Jin ZH, Ye ZF, Yang S, Sun YQ, Fei H. 2020. Exogenous enzymes as functional additives in finfish aquaculture. Aquac Nut. 26 (2): 213-224. DOI: https://doi.org/10.1111/anu.12995
Descargas
Archivos adicionales
Publicado
Número
Sección
Licencia
Derechos de autor 2026 Yamila E. Rodriguez, Juana C. del Valle, Ivana S. Friedman, María Victoria Laitano, Clara Liébana, Aldo N. Zanazzi, Analia V. Fernández-Gimenez
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-CompartirIgual 4.0.
Los autores de los artículos publicados en Marine and Fishery Sciences conservan los derechos de autor de sus artículos, a excepción de las imágenes de terceros y otros materiales añadidos por Marine and Fishery Sciences, que están sujetos a los derechos de autor de sus respectivos propietarios. Por lo tanto, los autores son libres de difundir y volver a publicar sus artículos, sujeto a los requisitos de los propietarios de derechos de autor de terceros y sujeto a que la publicación original sea completamente citada. Los visitantes también pueden descargar y reenviar artículos sujetos a los requisitos de citas. La capacidad de copiar, descargar, reenviar o distribuir cualquier material siempre está sujeta a los avisos de derechos de autor que se muestran. Los avisos de copyright deben mostrarse de manera prominente y no pueden borrarse, eliminarse u ocultarse, total o parcialmente. El autoalmacenamiento en servidores y repositorios de preimpresión está permitido para todas las versiones.
Esta revista ofrece a los autores una política de acceso abierto. Los usuarios pueden leer, descargar, copiar, distribuir, imprimir, buscar o vincular los textos completos de los artículos, o usarlos para cualquier otro propósito legal dentro de la licencia Creative Commons 4.0 (BY-NC-SA), sin solicitar permiso previo del editor o del autor. Esto está de acuerdo con la definición BOAI de acceso abierto.
