Effects of sodium metabisulfite on the activity and protease composition of the enzymatic extracts of Argentine red shrimp Pleoticus muelleri
DOI:
https://doi.org/10.47193/mafis.3512022010107Keywords:
Enzyme inhibition, shrimp, sodium metabisulfite, proteases.Abstract
The markets to which the Pleoticus muelleri Argentine red shrimp is exported require a product without the presence of melanosis. The preservative used in this species is sodium metabisulfite (MBS). So far, the effects that they could have on Argentine red shrimp proteases have not been studied. In this work, it is proposed to evaluate the effect of MBS on the activity and protease composition of the enzymatic extracts of P. muelleri. The acid and alkaline protease activity was determined, as well as the enzyme composition of the enzymatic extracts of Argentine red shrimps treated and untreated with MBS. Those extracts treated with MBS produced a significant reduction in protease activity (around 80%), both acidic and alkaline. In turn, the aspartic enzymes reduced their activity while the cysteine and serine enzymes of the trypsin type predominated. The enzyme extracts of P. muelleri treated with MBS show a reduction in protease activity and changes in their enzymatic composition, but still constitute a source of enzymes with the potential to be used for different biotechnological purposes.
Downloads
Metrics
Author Biographies
Clara Liebana, Departamento de Ciencias Marinas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata (UNMdP), Funes 3350, B7602AYL - Mar del Plata, Argentina - Instituto de Investigaciones Marinas y Costeras (IIMyC-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata (UNMdP), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), CC 1260. 7600 - Mar del Plata, Argentina
https://orcid.org/0000-0002-7062-8675Analía V. Fernández-Giménez, Departamento de Ciencias Marinas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata (UNMdP), Funes 3350, B7602AYL - Mar del Plata, Argentina - Instituto de Investigaciones Marinas y Costeras (IIMyC-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata (UNMdP), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), CC 1260. 7600 - Mar del Plata, Argentina
Nair de los Ángeles Pereira, Departamento de Ciencias Marinas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata (UNMdP), Funes 3350, B7602AYL - Mar del Plata, Argentina - Instituto de Investigaciones Marinas y Costeras (IIMyC-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata (UNMdP), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), CC 1260. 7600 - Mar del Plata, Argentina - Universidad Tecnológica Nacional (UTN), Regional Mar del Plata, Buque Pesquero Dorrego 281, B7600CLF - Mar del Plata. Argentina
References
Anson ML. 1938. The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin. J Gen Physiol. 22: 79-89.
Bovcon ND, Góngora ME, Marinao C, González Zevallos D. 2013. Composición de las capturas y descartes generados en la pesca de merluza común Merluccius hubbsi y langostino patagónico Pleoticus muelleri: un caso de estudio en la flota fresquera de altura del Golfo San Jorge, Chubut, Argentina. Rev Biol Mar Ocean. 48: 303-319.
Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72: 248-254.
Carranza-Espinal EO. 2014. Comparación de tres metodologías para la captación de sulfitos en camarones tratados con metabisulfito de sodio. Rev Cienc Tecnol. 14: 62-76.
Díaz-López MI, Martínez-Díaz T, Martínez-Moya M, Montero-García M, Gómez-Guillén J, Zamorano-Rodríguez y Martínez Álvarez O. 2003. Estudios de los agentes conservantes e inhibidores de la melanosis en crustáceos. Andalucía: Junta de Andalucía Consejería de Agricultura y Pesca. 188 p.
Ferrer OJ, Otwell S, Marshall MR. 1989. Effect of bisulfite on lobster shell phenoloxidase. J Food Sci. 54 (2): 478-480.
Friedman M, Gumbmann MR. 1986. Nutritional improvement of soy flour through inactivation of trypsin inhibitors by sodium sulfite. J Food Sci. 51: 1239.
García-Carreño FL. 1992a. The digestive proteases of langostilla Pleuronco desplanipes, Decapoda: Their partial characterization and the effect of fed on their composition. Comp Biochem Physiol. 103B: 575-578.
García-Carreño FL. 1992b. Protease inhibition in theory and practice. Biotech Educ. 3: 145-50.
Góngora ME, González-Zevallos D, Pettovello A, Mendia L. 2012. Caracterización de las principales pesquerías del golfo San Jorge Patagonia, Argentina. Lat Am J Aquat Res. 40 (1): 1-11.
Guerrero-Olazarán M, Castillo-Galván M, Gallegos-López JA, Fuentes-Garibay JA, Viader-Salvadó JM. 2019. Biochemical characterization of recombinant Penaeus vannamei trypsinogen. Comp Biochem Physiol B, Biochem Mol Biol. 238: 110337.
Lalithapriya U, Mariajenita P, Renuka V, Sudharsan K, Karthikeyan S, Sivarajan M, Murugan D, Sukumar M. 2019. Investigation of natural extracts and sodium bisulfite impact on thermal signals and physicochemical compositions of Litopenaeus vannamei during chilled storage. J Aquat Food Prod Technol. 28 (6): 1-15.
LaVoie MJ, Ostaszewski BL, Weihofen A, Schlossmacher MG, Selkoe DJ. 2005. Dopamine covalently modifies and functionally inactivates parkin. Nat Med. 11 (11): 1214-1221.
Lupano CE. 2013. Modificaciones de componentes de los alimentos: cambios químicos y bioquímicos por procesamiento y almacenamiento. La Plata: Editorial de la Universidad de La Plata (EDULP). 218 p.
MAGyP [Ministerio de Agricultura, Ganadería y Pesca]. 2021. Desembarques. https://www.magyp.gob.ar/sitio/areas/pesca_maritima/desembarques/lectura.php?imp=1&tabla=especie_flota_2020.
Moriondo Danovaro P, de la Garza J. 2019. Pesquería de langostino (Pleoticus muelleri). Resumen de la información biológica-pesquera reportada por los observadores a bordo en aguas de jurisdicción nacional. Temporada 2018. Inf Téc Of INIDEP Nº 9/2019. 14 p.
Otell SLG. 2010. Farm raised shrimp, good aquaculture practices for product quality and safety. Florida: Aquat Food Prod Progr. 71 p.
Pearl LH. 1987. The catalytic mechanism of aspartic proteinases. FEBS Lett. 214 (1): 8-12.
Pereira, NDLÁ, Fangio MF, Rodriguez YE, Bonadero MC, Harán NS, Fernández-Giménez AV. 2021. Characterization of liquid protein hydrolysates shrimp industry waste: analysis of antioxidant and microbiological activity, and shelf life of final product. J Food Process Preserv. 00: e15526.
Pereira NDLÁ, Fangio MF, Rodriguez YE, Garbari DF, Fernández-Giménez AV. 2020a. Obtención de hidrolizados proteicos a partir de desechos de la industria pesquera. En: Saraiva Pinheiro MS, editor. Oceanografía: develando la belleza, los misterios y los desafíos del mar. Curitiba: Editora Artemis. p. 99-110.
Pereira NDLÁ, Fernández-Giménez AV. 2016. Revalorización de subproductos de la pesca y su estado actual en Argentina y otros países de América Latina. Düsseldorf: Editorial Académica Española. 72 p.
Pereira NDLÁ, Fernández-Giménez AV. 2017. Exogenous enzymes in dairy technology: acidic proteases from processing discards of shrimp Pleoticus muelleri and their use as milk-clotting enzymes for cheese manufacture. Int J Food Sci Technol. 52: 341-347.
Pereira NDLÁ, Harán NS, Rodriguez YE, Fernández-Giménez AV. 2020b. Propuesta de un desarrollo industrial sostenible post covid19: coagulante lácteo a partir de la utilización de restos del procesamiento de especies pesqueras comerciales mediante tecnología verde. Latvia: Editorial Académica Española. 76 p.
Pereira NDLÁ, Rodriguez YE, Garbari D. 2018. Técnicas de obtención y utilización de enzimas de interés industrial provenientes de desechos del procesamiento del langostino argentino comercial Pleoticus muelleri. Latvia: Editorial Académica Española.
Pettovello AD, Boschi EE. 2016. La fauna acompañante del langostino patagónico (Pleoticus muelleri) en el Golfo San Jorge y adyacencias: análisis de alternativas de manejo. En: Boschi EE, editor. El Mar Argentino y sus recursos pesqueros. Tomo 6. Los crustáceos de interés pesquero y otras especies relevantes en los ecosistemas marinos. Mar del Plata: Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP). p. 89-94.
Rodríguez YE, Laitano MV, Pereira NA, López-Zavala AA, Haran NS, Fernández-Giménez AV. 2018. Exogenous enzymes in aquaculture: alginate and alginate-bentonite microcapsules for the intestinal delivery of shrimp proteases to Nile tilapia. Aquaculture. 490: 5-43.
Rodríguez YE, Pereira NA, Haran NS, Mallo JC, Fernández-Giménez AV. 2017. A new approach to fishery waste revalorization to enhance Nile tilapia (Oreochromis niloticus) digestion process. Aquacult Nutr. 23 (6): 1351-1361.
Rodríguez YE, Pereira NA, Laitano MV, Moreno P, Fernández-Giménez AV. 2021. Exogenous proteases from seafood processing waste as functional additives in rainbow trout aquaculture. Aquacult Res. 52 (9): 4350-4361. DOI: https://doi.org/10.1111/are.15272
Rodríguez-Casariego J, Perdomo-Morales R, Perera E. 2014. Purificación de isoformas de proteasas tipo tripsina de crustáceos. Rev Invest Mar. 32: 1-8.
Thein M, Michalke W. 1988. Bisulfite interacts with binding sites of the auxin-transport inhibitor N-1-naphthylphthalamic acid. Planta. 176 (3): 343-350.
Whitehead RE, Ferrer JV, Javitch JA, Justice, JB. 2001. Reaction of oxidized dopamine with endogenous cysteine residues in the human dopamine transporter. J Neurochem. 76: 1242-1251.
Xu ZH, Regenstein JM, Xie DD, Lu WJ, Ren XC, Yuan JJ, Mao LC. 2018. The oxidative stress and antioxidant responses of Litopenaeus vannamei to low temperature and air exposure. Fish Shellfish Immunol. 72: 564-571.
Zhang L, Sun XS. 2008. Effect of sodium bisulfite on properties of soybean glycinin. J Agr Food Chem. 56 (23): 11192-11197.
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Clara Liebana, Analía V. Fernández-Giménez, Nair de los Ángeles Pereira
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors of articles published in Marine and Fishery Sciences retain copyright on their articles, except for any third-party images and other materials added by Marine and Fishery Sciences, which are subject to copyright of their respective owners. Authors are therefore free to disseminate and re-publish their articles, subject to any requirements of third-party copyright owners and subject to the original publication being fully cited. Visitors may also download and forward articles subject to the citation requirements. The ability to copy, download, forward or otherwise distribute any materials is always subject to any copyright notices displayed. Copyright notices must be displayed prominently and may not be obliterated, deleted or hidden, totally or partially.
This journal offers authors an Open Access policy. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other legal purpose within the Creative Commons 4.0 license (BY-NC-SA), without asking prior permission from the publisher or the author. This is in accordance with the BOAI definition of Open Access.
