1
Marine and
Fishery Sciences
MAFIS
*
Length-weight relationships and mitochondrial COI variation of orange mud
crab (Scylla olivacea) across four mangrove localities in Peninsular Malaysia
1
1
1
Murak
Mohammad Ekramul Haque Muhammad Sajjad Ahmad
ABSTRACT.
Scylla olivacea
S. olivacea
=π=
==
Key words:
Relaciones talla-peso y variación del gen mitocondrial COI en el cangrejo naranja de fango
(Scylla olivacea) en cuatro localidades de manglar de Malasia peninsular
RESUMEN.
Scylla olivacea
S. olivacea
Marine and Fishery Sciences 40 (1): xxx-xxx (2027)
Scylla olivacea
S.
olivacea
Scylla
S. olivacea
c
=π=
==
Palabras clave:
Murak et al.: LWR and COI variation of Scylla olivacea
S. olivacea
Scylla
S.
olivacea
S. olivacea
S. olivacea
S. olivacea
Marine and Fishery Sciences 40 (1): xxx-xxx (2027)
S. olivacea
S. olivacea
S. olivacea
Sampling and preservation
S. olivacea
=
Scylla
Scylla olivacea=
'" '"
'" '"
'" '"
'" '"
Murak et al.: LWR and COI variation of Scylla olivacea
Length-weight relationship (LWR) analysis
BW = aCWb
a b
BW=abCW
ab
BWCW
b=
Scylla olivacea
=
===
99.00 100.00 101.00 102.00 103.00 104.00
100.00 101.00 102.00 103.00 104.00
TDM
SWM
SMMF
LMP
100 km500
99.004.00 6.00
4.006.00
Marine and Fishery Sciences 40 (1): xxx-xxx (2027)
6
DNA extraction and quantication
Polymerase chain reaction (PCR) amplication
and sequencing
μ μ
μ μ
μμμ
×
μ
Sequence alignment and editing
Murak et al.: LWR and COI variation of Scylla olivacea
Demographic history and population expansion
S. oliva-
cea
Fs
θθ1
τ
RI
>
Length-weight relationship
==
=
R=
==
==
Marine and Fishery Sciences 40 (1): xxx-xxx (2027)
b=
Genetic diversity
Haplotype distribution
S.
olivacea
Genetic diversity indices
S. oliva-
cea
Scylla olivacea
Location
Kedah
Kelantan
Perak
Terengganu
SWM, Terengganu
y = 3.6937x - 5.0115
R = 0.9711
2
TDM, Kelantan
y = 2.1602x - 2.0391
R = 0.8091
2
LMP, Perak
y = 3.002x - 3.6934
R = 0.9388
2
SMMF, Kedah
y = 3.5623x - 4.7487
R = 0.9702
2
2.6
2.4
2.2
2.0
1.90 1.95 2.00
Log carapace width (mm)
10
2.05
Log body weight (g)
10
Murak et al.: LWR and COI variation of Scylla olivacea
Scylla olivacea
Marine and Fishery Sciences 40 (1): xxx-xxx (2027)
π
=
=
=
Population genetic structure
Median joining network
Murak et al.: LWR and COI variation of Scylla olivacea 11
Maximum likelihood tree
S.
olivacea
Analysis of Molecular Variance (AMOVA)
S.ol-
ivacea
=
Scylla olivacea
π
Marine and Fishery Sciences 40 (1): xxx-xxx (2027)
=
Pairwise population dierentiation
=
Scylla olivacea
==
==
Hap21
Hap35
Hap9
Hap11
Hap20
Hap7
Hap44
Hap43
Hap3
Hap1
Hap24
Hap25
Hap54
Hap2
Hap34
Hap32
Hap33
Hap27
Hap6
Hap28
Hap12
Hap26
Hap15
Hap46
Hap56
Hap14
Hap58
Hap38
Hap55
Hap22
Hap23
Hap50
Hap57
Hap19
Hap41
Hap39
Hap51
Hap42
Hap52
Hap31
Hap37
Hap47
Hap36
Hap29
Hap5
Hap13
Hap4
Hap18
Hap17
Hap40
Hap30
Hap48
Hap8
Hap45
Hap10
Hap16
Hap49
Hap53
ST
Kel
Kd
PR
10 samples
1 sample
Murak et al.: LWR and COI variation of Scylla olivacea
Scylla olivacea
Hap21
Hap35
Hap9
Hap11
Hap20
Hap7
Hap44
Hap43
Hap3
Hap1
Hap24
Hap25
Hap54
Hap2
Hap34
Hap32
Hap33
Hap27
Hap6
Hap28
Hap12
Hap26
Hap15
Hap46
Hap56
Hap14
Hap58
Hap38
Hap55
Hap22
Hap23
Hap50
Hap57
Hap19
Hap41
Hap39
Hap51
Hap42
Hap52
Hap31
Hap37
Hap47
Hap36
Hap29
Hap5
Hap13
Hap4
Hap18
Hap17
Hap40
Hap30
Hap48
Hap8
Hap45
Hap10
Hap16
Hap49
Hap53
0.05
72
80
78
70
72
Marine and Fishery Sciences 40 (1): xxx-xxx (2027)
=
α=
Historical demography
Neutrality tests
>
>
Fs
k
=α= Fs
Mismatch distribution analysis
τ
θ
=
Scylla olivacea
±
==
===
± ± ±
± ±
±
Murak et al.: LWR and COI variation of Scylla olivacea
θ1
θθ1
τ
Goodness-of-t to the expansion model
RI
RI
Pairwise nucleotide dierences
FsScylla olivacea
k===
==
Fs
<
<
<
<
θθ1τ
Scylla olivacea
θθ1
τ=
==
=
θ θ τ
Marine and Fishery Sciences 40 (1): xxx-xxx (2027)
16
RI
Scylla olivacea
0.15
0.10
0.05
0.00
0510 15
Pairwise differences
Frequency
Expected
Observed
RI
=Scylla olivacea
≥
===
=
RI
Murak et al.: LWR and COI variation of Scylla olivacea
Body-size allometry
S. olivacea
Mitochondrial COI genetic diversity and popu-
lation structure
=
π≈
Marine and Fishery Sciences 40 (1): xxx-xxx (2027)
S. ol-
ivacea
==
S. oli-
vacea
Relationship between body-size allometry and
genetic structure
S.
olivacea
Historical demographic patterns
Fs
Murak et al.: LWR and COI variation of Scylla olivacea
Fs
Implications and limitations
S. olivacea
S. olivacea
b
π
Marine and Fishery Sciences 40 (1): xxx-xxx (2027)
Ethics statement
Funding
Declaration of generative AI in scientic writing
Declaration of competing interest
Data availability statement
Author contributions
Murak et al.: LWR and COI variation of Scylla olivacea
Scylla
Scylla olivacea
Scylla
olivacea
Scylla
Scylla
Scylla olivacea
Scylla
Scylla olivacea
Marine and Fishery Sciences 40 (1): xxx-xxx (2027)
Scylla olivacea
Scylla
Scylla paramamosain
